ANA Immunoblot Test Price in Bhubaneswar

Test Description

ANA Immunoblot (also called ANA Profile or ENA Profile by immunoblot) is a line immunoassay (LIA) that detects IgG antibodies against a panel of specific nuclear and cytoplasmic autoantigens associated with systemic autoimmune rheumatic diseases (SARDs). The panel typically includes antibodies against: dsDNA (double-stranded DNA), Sm (Smith antigen, core component of snRNP), SS-A/Ro 52 kD and 60 kD, SS-B/La, Scl-70 (topoisomerase I), CENP-B (centromere protein B), Jo-1 (histidyl-tRNA synthetase), PM-Scl, Mi-2, PCNA, ribosomal P protein, and histones. Each specificity is associated with particular conditions: anti-dsDNA and anti-Sm are highly specific for SLE; anti-SS-A/Ro and anti-SS-B/La for Sjögren's syndrome and neonatal lupus; anti-Scl-70 for diffuse cutaneous systemic sclerosis; anti-CENP-B for limited cutaneous SSc (CREST syndrome); anti-Jo-1 for polymyositis/dermatomyositis and antisynthetase syndrome. ANA Immunoblot provides specific autoantibody identification beyond the initial ANA screening (IFA or ELISA), enabling disease subtype classification, prognostic stratification, and identification of overlap syndromes. It is used as a reflex or confirmatory test after a positive ANA screen and in clinical suspicion for specific connective tissue disease.